WebTHP1-Lucia™ ISG cells express the secreted luciferase (Lucia) reporter gene under the control of an IRF-inducible promoter. This composite promoter is comprised of five IFN … WebDownload scientific diagram PMA, LPS and IFN-γ treatment promoted THP-1 cells differentiation to M1 macrophages. A, B. The effect of PMA, LPS and IFN-γ treatment on …
M1 and M2 macrophages derived from THP-1 cells ... - BMC Cancer
WebTHP1-Dual ™ KO-IFNAR2 and THP1-Dual ™ cells can be used to study the role of IFNAR2 by monitoring IRF-induced Lucia luciferase activity. THP1-Dual ™ KO-IFNAR2 cells are … WebSep 15, 1992 · We have studied two distinct pathways in the differentiation of a human monocytic cell line, THP-1: one being induced by IFN-gamma and the other by 1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3). In THP-1 cells, IFN-gamma induces cell surface expression of HLA-DR and CD54 and production of IL-1 beta, TNF-alpha, and IL-6. paldean bisharp
THP1-Dual™ KO-IRF3 cells NF-κB & IFN reporter …
WebFeb 26, 2024 · The proinflammatory cytokines interleukin (IL)-1β and IL-18 are products of activation of the inflammasome, an innate sensing system, and important in the pathogenesis of herpes simplex virus type 1 (HSV-1). The release of IL-18 and IL-1β from monocytes/macrophages is critical for protection from HSV-1 based on animal models of … WebMar 31, 2024 · IFN-γ is known to enhance presentation of tumor-associated antigens by upregulating the immunoproteosome and catalyzing nondestructive cleavage, thereby enhancing major histocompatibility complex peptide surface expression leading to increased T-cell recognition and cytotoxicity. 10, 26-30 To determine whether IFN-γ … WebNov 17, 2024 · When PMA-treated THP-1 cells were incubated with IFN-λ4 for 48 h, no effect was observed on TNF-α and IL-6 cytokine secretion (Fig. 1B), suggesting that the recombinant protein was free from any endotoxin contamination. Flow cytometry analysis revealed that PMA-treated THP-1 cells 52 had increased IFN-LR1 expression (Fig. 1C). paldea legendary pokemon