2024 Hoechst 33342 structure

Hoechst 33342 structure

Author: eyps

August undefined, 2024

NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish … NettetDescription. Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570). Apoptosis, Cell Analysis, Cell Cycle, Cell ...

NucBlue™ Live ReadyProbes™ Reagent (Hoechst 33342)

Nettetjournals.asm.org NettetBisbenzimide C27H28N6O CID 1464 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, … joanie greggains age and birthday

Discovery of DNA dyes Hoechst 34580 and 33342 as good

Nettet18. apr. 2024 · Hoechst 33342 and 33258 are the most commonly used bis-benzimides with similar excitation spectra; however, the former is significantly more permeable than … NettetIts uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. For Research Use Only. Not for use in diagnostic … NettetHoechst 33342 Staining Dye Solution (ab228551) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, … insto4irwf

Effect of vascular marker Hoechst 33342 on tumour perfusion and ...

Nettet29. jun. 2024 · Structural optimization of aureusidin gave rise to a derivative named CNQX. LPS was used to induce inflammation in intestinal macrophages; flow cytometry, PI staining and Hoechst 33342 staining were used to detect the apoptotic level of macrophages; enzyme‐linked immunosorbent assay ... NettetStructure Search. Search Within. Products Technical Documents Site Content Papers Genes Chromatograms. Product Category. dyes (2) Brand. Sigma-Aldrich (4) Formula Weight. Physical Form. ... red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. insto campur spriteNettetHoechst 33258, pentahydrate (bis-benzimide) (MW 623.96) (H3569) 10 mL 10 mg/mL (16.0 mM) solution in water Store at 2–6°C Protect from light • • When stored as directed, product is stable for 6 months. Hoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from light • • inst neymar

"NettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the … " - Hoechst 33342 structure

Hoechst 33342 structure

Photothermal exposure of polydopamine-coated branched Au–Ag ...

Nettet15. des. 2024 · Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain. Storage: protect from light. ... Hoechst 33342 trihydrochloride Chemical Structure. CAS No. : 875756-97-1. Size Price Stock Quantity; Free Sample (0.1 - 0.5 mg) Apply Now : 50 mg USD 50 In-stock

Did you know?

NettetStore at -20°C Hoechst 33342 rev. 08/24/18 n 25 mg IF-IC, IF-F, IF-P, F All Description: Hoechst 33342 (bisBenzimide H33342 trihydrochloride) is supplied as a lyophilized … NettetScheme 1 Structure of L. 1 实验部分 1.1 实验试剂与仪器. RPMI1640培养基、DMEM培养基、胰蛋白酶、胎牛血清、溴化噻唑蓝四氮唑(噻唑蓝，MTT)、Hoechst 33342染色试剂、DCFH-DA探针、JC-1线粒体膜电位检测试剂盒均购自索莱宝公司，二甲基亚砜(DMSO)购自 …

Nettet18. apr. 2024 · Although often overlooked in the biomedical literature, the generic name “Hoechst” can refer to any of related bisbenzimide dyes (Hoechst 33258, Hoechst 33342 and Hoechst 34580 (Figure 1a and Table 1) [3,4]. They were developed by the German company Hoechst AG in the early 1970s and are being used for DNA staining ever since. NettetThe fluorescent stain Hoechst 33342 (H33342) has been employed extensively as an in vivo marker of functional tumour vasculature. We have found that H33342 causes a transient, dose-dependent decrease in tumour red blood cell (RBC) flow in SCCVII tumours as measured using laser Doppler flowmetry.

NettetBisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in … NettetHoechst 33342 Staining Protocol λ Ex /λ Em (with DNA) = 350/461 nm MW: 561.93 (in H 2 O); 615.98 (trihydrochloride trihydrate) Molecular formula: C 27 H 31 Cl 3 N 6 O CAS number: 23491-52-3 Recommended staining concentration: 1 ug/mL Hoechst 33342, trihydrochloride trihydrate Interested in Ordering? Check Out Our New Lab Start-Up …

Nettet10. jan. 2024 · Hoechst 33258 , Hoechst 33342, and Hoechst 34580 are Bisbenzimides with intercalating tendency to A-T rich areas. Similar to DAPI they are excited by UV-light and have an emission maximum at 455 nm which is shifted to 510–540 nm in an unbound condition. Hoechst stains are also cell permeable and can therefore be used in fixed …

Nettet5. nov. 2015 · The fluorescent dye Hoechst 33342 is a substrate for many multidrug transporters and is extruded by efflux pumps in microbial and mammalian cells. ... Alemán C, Namba AM, Casanovas J. Acid-base and electronic structure-dependent properties of Hoechst 33342. J Biomol Struct Dyn 2005; 23: 29–36. pmid:15918674 . View Article instn saclayNettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … instn saclay adresseNettetHoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when ... It is also available in solution (H-3570). Apoptosis, Cell Analysis, Cell Cycle, Cell Proliferation, Cell Structure, Cell Tracing & Tracking, Cell Viability & Cytotoxicity, Cell Viability, Proliferation & Function, Cellular Imaging, Chromatin ... joanie ivy arrestedNettetDescription. Thermo Scientific™ Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques. Hoechst 33342 (2'- [4-ethoxyphenyl]-5- [4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell ... joanie hampton williams facebookNettetビスベンズイミド H 33342 三塩酸塩. Synonym (s): 2′- (4-エトキシフェニル)-5- (4-メチル-1-ピペラジニル)-2,5′-ビ-1H-ベンゾイミダゾール三塩酸塩, HOE 33342, Hoechst … joanie hope anchorage akNettetHoechst 33342 is more cell permeable than Hoechst 33258 (sc-391053) due to an extra ethyl group in the structure increasing lipophilicity. Hoechst dyes may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI (sc-3598). Also commonly used to visualize nuclei and mitochondria. joanie heighes realtorNettet20. feb. 2015 · Other properties listed for this fluorescent dye include the physical form, solubility, melting point, boiling point, pKa, absorption maxima, emission maxima, molar extinction coefficient and quantum yield. The chapter also includes references on the use, properties, and safety/toxicity of Hoechst 33342 on humans, animals and the … instnwnd.sql